Other

Part:BBa_K277032

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-20)


3L.3_23.B1.06

3L.3_23.B1.06 is 750 bases long and is cloned into the pGem-T vector.

3L.3_23.B1.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.06 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

reverse_primer YCL054W_tagr1v1 (648..675)

mutation_affecting_coding_sequence YCL054W_re_remove_BlpI (274..282) removal of BlpI

forward_primer YCL054W_tagf1v1 (243..270)

gene YCL054W (-1181..+1344) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants

Sequence (the first 750 bases correspond to coordinates 22834..23583 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 712
    Illegal XbaI site found at 333
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 712
    Illegal PstI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 712
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 712
    Illegal XbaI site found at 333
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 712
    Illegal XbaI site found at 333
    Illegal PstI site found at 53
    Illegal AgeI site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None